IBD IN VITRO MODELS

Intestinal Biotech Development is using in vitro tools to evaluate the potential anti-inflammatory properties of compounds (natural or synthetic) or probiotics.

Cells

Different types of cells could be used to evaluate the effects of compounds on inflammation. The choice of cells will be determined with respect to the topic of the study we will carry out:

• Cell lines: HT-29, Caco-2, Lymphocytes, macrophages
• Cells extracted from tissues (epithelial cells from colon, for example), PBMC
• Biopsies from tissues

The anti-inflammatory effects of compounds could be tested in cells pre-stimulated or not by LPS (a bacterial pro-inflammatory product), TNF (a pro-inflammatory cytokine)

Parameters studied

Study of pro- and anti-inflammatory mediators (cytokines) mRNA level by Real Time PCR:

• Protein level by ELISA, Western Blot, Immunohistochemistry
• Protein level by dosage of the myeloperoxydase (MPO) activity Study of specific therapeutic targets mRNA level by Real Time PCR
• Protein level by Western Blot, Immunohistochemistry